Difference between revisions of "DNA Sequencing"

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There are several new technologies for whole genome sequencing in addition to the classic workhorse of Sanger sequencing.   
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There are several new technologies for whole genome sequencing in addition to the classic workhorse of Sanger sequencing.  This area is rapidly changing and it is challenging to stay on top of the current technology.   
  
Here are the big four technologies as of July 2013[http://ngs-expert.com/2013/07/11/amplicon-sequencing-strategy-what-is-your-technology-of-choice/]
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Here are some major technologies being used:
 
*[[Sanger Sequencing]]
 
*[[Sanger Sequencing]]
 
*[[Illumina MiSeq]]
 
*[[Illumina MiSeq]]
 
*[[Ion Torrent]]
 
*[[Ion Torrent]]
 
*[[Roche 454]]
 
*[[Roche 454]]
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*[[Pacific Biosciences RS]]
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=To Do=
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Quail, M. A., Smith, M., Coupland, P., Otto, T. D., Harris, S. R., Connor, T. R., ... & Gu, Y. (2012). A tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers. BMC genomics, 13(1), 341.
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Loman, N. J., Misra, R. V., Dallman, T. J., Constantinidou, C., Gharbia, S. E., Wain, J., & Pallen, M. J. (2012). Performance comparison of benchtop high-throughput sequencing platforms. Nature biotechnology, 30(5), 434-439.
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Nagarajan, N., & Pop, M. (2013). Sequence assembly demystified. Nature Reviews Genetics, 14(3), 157-167.
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Shendure, J., & Aiden, E. L. (2012). The expanding scope of DNA sequencing. Nature biotechnology, 30(11), 1084-1094.

Latest revision as of 00:42, 19 July 2014

There are several new technologies for whole genome sequencing in addition to the classic workhorse of Sanger sequencing. This area is rapidly changing and it is challenging to stay on top of the current technology.

Here are some major technologies being used:

To Do

Quail, M. A., Smith, M., Coupland, P., Otto, T. D., Harris, S. R., Connor, T. R., ... & Gu, Y. (2012). A tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers. BMC genomics, 13(1), 341.

Loman, N. J., Misra, R. V., Dallman, T. J., Constantinidou, C., Gharbia, S. E., Wain, J., & Pallen, M. J. (2012). Performance comparison of benchtop high-throughput sequencing platforms. Nature biotechnology, 30(5), 434-439.

Nagarajan, N., & Pop, M. (2013). Sequence assembly demystified. Nature Reviews Genetics, 14(3), 157-167.

Shendure, J., & Aiden, E. L. (2012). The expanding scope of DNA sequencing. Nature biotechnology, 30(11), 1084-1094.