View source for ExoSAP Reaction

The ExoSAP reaction "removes" the dNTP's and [[PCR primers]] from the [[PCR product]] to prepare it for [[Sanger Sequencing]].  It contains [[Exonuclease I]] and [[Shrimp Alkaline Phosphatase]] that chop up single stranded primers and inactivate dNTP's so they do not interfere with the sequencing reaction. The mixture is: 
:10 μl PCR product
:3 μl ExoSAP (which is 1 μl of Exonuclease I and 2 μl of Shrimp Alkaline Phosphatase)

The mixture is heated to 37 C for 15 min then 80 C for 15 min.  This is under the program "EXSAP" on the thermocycler.

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