Difference between revisions of "Lysogeny Broth (LB)"
From Genetics Wiki
(Created page with "Lysogeny Broth (LB) is made up of: :Casein Peptone 10 g/l :Yeast Extract 5 g/l :Sodium Chloride 10 g/l Liquid LB mix is 2.5 g per 100 ml solution. Ready made powder can be o...") |
|||
Line 4: | Line 4: | ||
:Sodium Chloride 10 g/l | :Sodium Chloride 10 g/l | ||
− | Liquid LB mix is 2.5 g per 100 ml solution. | + | Liquid LB mix is 2.5 g per 100 ml solution. Freshly made mix without antibiotics should be autoclaved at 121 C for 20 min or placed in boiling water for 30 min to sterilize. |
+ | |||
+ | Pre-mixed powder can be ordered, e.g., (USB/Affymetrix stock #75854). | ||
+ | |||
+ | =What Links Here= | ||
+ | {{Special:WhatLinksHere/{{FULLPAGENAME}}}} |
Revision as of 08:17, 17 July 2018
Lysogeny Broth (LB) is made up of:
- Casein Peptone 10 g/l
- Yeast Extract 5 g/l
- Sodium Chloride 10 g/l
Liquid LB mix is 2.5 g per 100 ml solution. Freshly made mix without antibiotics should be autoclaved at 121 C for 20 min or placed in boiling water for 30 min to sterilize.
Pre-mixed powder can be ordered, e.g., (USB/Affymetrix stock #75854).