Difference between revisions of "Lysogeny Broth (LB)"
From Genetics Wiki
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Lysogeny Broth (LB) is made up of: | Lysogeny Broth (LB) is made up of: | ||
| − | :Casein Peptone 10 g/l | + | :[[Casein Peptone]] 10 g/l |
| − | :Yeast Extract 5 g/l | + | :[[Yeast Extract]] 5 g/l |
| − | :Sodium Chloride 10 g/l | + | :[[Sodium Chloride]] 10 g/l |
| − | Liquid LB mix is 2.5 g per 100 ml solution. Freshly made mix without antibiotics should be autoclaved | + | Liquid LB mix is 2.5 g per 100 ml solution. Freshly made mix without antibiotics should be autoclaved at 121 C for 20 min or placed in boiling water for 30 min to sterilize. |
Pre-mixed powder can be ordered, e.g., (USB/Affymetrix stock #75854). | Pre-mixed powder can be ordered, e.g., (USB/Affymetrix stock #75854). | ||
Revision as of 08:22, 17 July 2018
Lysogeny Broth (LB) is made up of:
- Casein Peptone 10 g/l
- Yeast Extract 5 g/l
- Sodium Chloride 10 g/l
Liquid LB mix is 2.5 g per 100 ml solution. Freshly made mix without antibiotics should be autoclaved at 121 C for 20 min or placed in boiling water for 30 min to sterilize.
Pre-mixed powder can be ordered, e.g., (USB/Affymetrix stock #75854).
