ExoSAP Reaction
From Genetics Wiki
Revision as of 08:53, 17 July 2018 by Floyd (talk | contribs) (Created page with "The ExoSAP reaction "removes" the dNTP's and PCR primers from the PCR product to prepare it for sequencing. It contains Exonuclease I and Shrimp Alkaline Phosphatase...")
The ExoSAP reaction "removes" the dNTP's and PCR primers from the PCR product to prepare it for sequencing. It contains Exonuclease I and Shrimp Alkaline Phosphatase that remove single stranded primers and inactivate dNTP's. The mixture is:
- 10 μl PCR product
- 3 μl ExoSAP (which is 1 μl of Exonuclease I and 2 μl of Shrimp Alkaline Phosphatase)
The mixture is heated to 37 C for 15 min then 80 C for 15 min. This is under the program "EXSAP" on the thermocycler.
