Pipetting

There are two main but related methods to pipetting, micropipetting for volumes of 1 ml or less and regular pipetting for volumes greater than 1 ml.

micropipetting

Volumes in the 1 µl – 20 µl range should be transferred with a P20 micropipette.

Volumes in the 20 µl – 200 µl range should be transferred with a P200 micropipette.

Volumes in the 200 µl – 1000 µl range should be transferred with a P1000 micropipette.

In general micropipettes work best in the middle of their range (half of their "number", e.g., 100 µl for a p200).

Volumes less than 1 µl can be tricky to work with. Use a P10 micropipette instead of a P20 and/or extended pipette tips if you have them.