Chromoprotein Mutagenesis

This has not been tried yet. I have been thinking of a way include generating mutations in the lab.

The general idea I have in mind is using PCR mutagenesis to make haphazard changes to chromoproteins, clone these into E. coli, select clones with interesting novel phenotypes, then sequence the insert to find the mutation responsible.

Literature

Liljeruhm, J., Funk, S. K., Tietscher, S., Edlund, A. D., Jamal, S., Wistrand-Yuen, P., ... & Törnblom, V. (2018). Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. Journal of Biological Engineering, 12(1), 8; https://jbioleng.biomedcentral.com/articles/10.1186/s13036-018-0100-0 ; https://scholar.google.com/scholar?cluster=17927173279619010872