PCR

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For a 25 ul reaction add:

H2O 10.5 µl
Taq 2X Master Mix 12.5 µl
Forward primer 0.5 ul 10 µM solution
Reverse primer 0.5 ul 10 µM solution
DNA template 1 µl

General PCR cycling conditions are:

An initial 95 C 30 s
Then 30 cycles of:
95 C 20 s melting
annealing temperature (this varies with the primers used, usually between 55 C and 65 C although some primers can be lower) 40s
70 C 1 m extension (this can be raised to 5 m for several kbp long segments)
With a final extension of 70 C for 5 minutes.