Agarose Gels

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Revision as of 04:32, 28 July 2018 by Floyd (talk | contribs) (Simple 1% Ethidium Bromide (EtBr) Gel)

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Simple 1% Ethidium Bromide (EtBr) Gel

1 g Agarose

100 mL 1X TAE

Mix agarose into the TAE buffer in a microwaveable clear container. Microwave in 30 s to 1 min intervals, handle the container with a silicone glove, swirling between each step to mix the agarose to help it dissolve. Be careful of flash boiling and the hot liquid suddenly splattering or spilling over.

Once the agarose is fully dissolved and clear let it cool a few minutes and then add EtBr and swirl to mix.

2.5 μl EtBr

...pouring the gel into a mold ...

Notes

Different gel concentrations ...

Gel Stains (potentially optional) ...

Used For

Electrophoresis

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