Difference between revisions of "Chromoprotein Mutagenesis"
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This has not been tried yet. I have been thinking of a way include generating mutations in the lab. | This has not been tried yet. I have been thinking of a way include generating mutations in the lab. | ||
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| + | The general idea I have in mind is using PCR mutagenesis to make haphazard changes to chromoproteins, clone these into E. coli, select clones with interesting novel phenotypes, then sequence the insert to find the mutation responsible. | ||
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| + | =Literature= | ||
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| + | :Liljeruhm, J., Funk, S. K., Tietscher, S., Edlund, A. D., Jamal, S., Wistrand-Yuen, P., ... & Törnblom, V. (2018). Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. ''Journal of Biological Engineering'', 12(1), 8; https://jbioleng.biomedcentral.com/articles/10.1186/s13036-018-0100-0 ; https://scholar.google.com/scholar?cluster=17927173279619010872 | ||
Revision as of 14:22, 18 July 2018
This has not been tried yet. I have been thinking of a way include generating mutations in the lab.
The general idea I have in mind is using PCR mutagenesis to make haphazard changes to chromoproteins, clone these into E. coli, select clones with interesting novel phenotypes, then sequence the insert to find the mutation responsible.
Literature
- Liljeruhm, J., Funk, S. K., Tietscher, S., Edlund, A. D., Jamal, S., Wistrand-Yuen, P., ... & Törnblom, V. (2018). Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. Journal of Biological Engineering, 12(1), 8; https://jbioleng.biomedcentral.com/articles/10.1186/s13036-018-0100-0 ; https://scholar.google.com/scholar?cluster=17927173279619010872
