Difference between revisions of "Chromoprotein Mutagenesis"
From Genetics Wiki
| Line 1: | Line 1: | ||
This has not been tried yet. I have been thinking of a way include generating mutations in the lab. | This has not been tried yet. I have been thinking of a way include generating mutations in the lab. | ||
| − | The general idea I have in mind is using PCR mutagenesis to make haphazard changes to chromoproteins, clone these into E. coli, select clones with interesting novel phenotypes, then sequence the insert to find the mutation responsible. | + | The general idea I have in mind is using PCR mutagenesis (error prone PCR) to make haphazard changes to chromoproteins, clone these into E. coli, select clones with interesting novel phenotypes, then sequence the insert to find the mutation responsible. |
=Literature= | =Literature= | ||
:Liljeruhm, J., Funk, S. K., Tietscher, S., Edlund, A. D., Jamal, S., Wistrand-Yuen, P., ... & Törnblom, V. (2018). Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. ''Journal of Biological Engineering'', 12(1), 8; https://jbioleng.biomedcentral.com/articles/10.1186/s13036-018-0100-0 ; https://scholar.google.com/scholar?cluster=17927173279619010872 | :Liljeruhm, J., Funk, S. K., Tietscher, S., Edlund, A. D., Jamal, S., Wistrand-Yuen, P., ... & Törnblom, V. (2018). Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. ''Journal of Biological Engineering'', 12(1), 8; https://jbioleng.biomedcentral.com/articles/10.1186/s13036-018-0100-0 ; https://scholar.google.com/scholar?cluster=17927173279619010872 | ||
| + | |||
| + | :Roberts, T. M., Rudolf, F., Meyer, A., Pellaux, R., Whitehead, E., Panke, S., & Held, M. (2016). Identification and characterisation of a pH-stable GFP. ''Scientific Reports'', 6, 28166; https://www.nature.com/articles/srep28166 ; https://scholar.google.com/scholar?cluster=13704240519438793468 | ||
| + | |||
| + | :Ruller, R., Silva‐Rocha, R., Silva, A., Cruz Schneider, M. P., & Ward, R. J. (2011). A practical teaching course in directed protein evolution using the green fluorescent protein as a model. ''Biochemistry and Molecular Biology Education'', 39(1), 21-27; https://iubmb.onlinelibrary.wiley.com/doi/full/10.1002/bmb.20430 ; https://scholar.google.com/scholar?cluster=10821652129227038353 | ||
Revision as of 14:27, 18 July 2018
This has not been tried yet. I have been thinking of a way include generating mutations in the lab.
The general idea I have in mind is using PCR mutagenesis (error prone PCR) to make haphazard changes to chromoproteins, clone these into E. coli, select clones with interesting novel phenotypes, then sequence the insert to find the mutation responsible.
Literature
- Liljeruhm, J., Funk, S. K., Tietscher, S., Edlund, A. D., Jamal, S., Wistrand-Yuen, P., ... & Törnblom, V. (2018). Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. Journal of Biological Engineering, 12(1), 8; https://jbioleng.biomedcentral.com/articles/10.1186/s13036-018-0100-0 ; https://scholar.google.com/scholar?cluster=17927173279619010872
- Roberts, T. M., Rudolf, F., Meyer, A., Pellaux, R., Whitehead, E., Panke, S., & Held, M. (2016). Identification and characterisation of a pH-stable GFP. Scientific Reports, 6, 28166; https://www.nature.com/articles/srep28166 ; https://scholar.google.com/scholar?cluster=13704240519438793468
- Ruller, R., Silva‐Rocha, R., Silva, A., Cruz Schneider, M. P., & Ward, R. J. (2011). A practical teaching course in directed protein evolution using the green fluorescent protein as a model. Biochemistry and Molecular Biology Education, 39(1), 21-27; https://iubmb.onlinelibrary.wiley.com/doi/full/10.1002/bmb.20430 ; https://scholar.google.com/scholar?cluster=10821652129227038353
