Difference between revisions of "ExoSAP Reaction"
From Genetics Wiki
(Created page with "The ExoSAP reaction "removes" the dNTP's and PCR primers from the PCR product to prepare it for sequencing. It contains Exonuclease I and Shrimp Alkaline Phosphatase...") |
(No difference)
|
Revision as of 08:53, 17 July 2018
The ExoSAP reaction "removes" the dNTP's and PCR primers from the PCR product to prepare it for sequencing. It contains Exonuclease I and Shrimp Alkaline Phosphatase that remove single stranded primers and inactivate dNTP's. The mixture is:
- 10 μl PCR product
- 3 μl ExoSAP (which is 1 μl of Exonuclease I and 2 μl of Shrimp Alkaline Phosphatase)
The mixture is heated to 37 C for 15 min then 80 C for 15 min. This is under the program "EXSAP" on the thermocycler.
