Difference between revisions of "Lysogeny Broth (LB)"
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(Created page with "Lysogeny Broth (LB) is made up of: :Casein Peptone 10 g/l :Yeast Extract 5 g/l :Sodium Chloride 10 g/l Liquid LB mix is 2.5 g per 100 ml solution. Ready made powder can be o...") |
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Revision as of 08:16, 17 July 2018
Lysogeny Broth (LB) is made up of:
- Casein Peptone 10 g/l
- Yeast Extract 5 g/l
- Sodium Chloride 10 g/l
Liquid LB mix is 2.5 g per 100 ml solution. Ready made powder can be ordered (USB/Affymetrix stock #75854). Freshly made mix without antibiotics should be autoclaved at 121 C for 20 min or placed in boiling water for 30 min to sterilize.