Difference between revisions of "Lysogeny Broth (LB)"

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(Created page with "Lysogeny Broth (LB) is made up of: :Casein Peptone 10 g/l :Yeast Extract 5 g/l :Sodium Chloride 10 g/l Liquid LB mix is 2.5 g per 100 ml solution. Ready made powder can be o...")
 
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:Sodium Chloride 10 g/l
 
:Sodium Chloride 10 g/l
  
Liquid LB mix is 2.5 g per 100 ml solution. Ready made powder can be ordered (USB/Affymetrix stock #75854).  Freshly made mix without antibiotics should be autoclaved  at 121 C for 20 min or placed in boiling water for 30 min to sterilize.
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Liquid LB mix is 2.5 g per 100 ml solution. Freshly made mix without antibiotics should be autoclaved  at 121 C for 20 min or placed in boiling water for 30 min to sterilize.
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Pre-mixed powder can be ordered, e.g., (USB/Affymetrix stock #75854). 
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=What Links Here=
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Revision as of 08:17, 17 July 2018

Lysogeny Broth (LB) is made up of:

Casein Peptone 10 g/l
Yeast Extract 5 g/l
Sodium Chloride 10 g/l

Liquid LB mix is 2.5 g per 100 ml solution. Freshly made mix without antibiotics should be autoclaved at 121 C for 20 min or placed in boiling water for 30 min to sterilize.

Pre-mixed powder can be ordered, e.g., (USB/Affymetrix stock #75854).

What Links Here