Difference between revisions of "Lysogeny Broth (LB)"

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Lysogeny Broth (LB) is made up of:
 
Lysogeny Broth (LB) is made up of:
:Casein Peptone 10 g/l
+
:[[Casein Peptone]] 10 g/l
:Yeast Extract 5 g/l
+
:[[Yeast Extract]] 5 g/l
:Sodium Chloride 10 g/l
+
:[[Sodium Chloride]] 10 g/l
  
Liquid LB mix is 2.5 g per 100 ml solution. Freshly made mix without antibiotics should be autoclaved at 121 C for 20 min or placed in boiling water for 30 min to sterilize.
+
Liquid LB mix is 2.5 g per 100 ml solution. Freshly made mix without antibiotics should be autoclaved at 121 C for 20 min or placed in boiling water for 30 min to sterilize.
  
 
Pre-mixed powder can be ordered, e.g., (USB/Affymetrix stock #75854).   
 
Pre-mixed powder can be ordered, e.g., (USB/Affymetrix stock #75854).   

Revision as of 08:22, 17 July 2018

Lysogeny Broth (LB) is made up of:

Casein Peptone 10 g/l
Yeast Extract 5 g/l
Sodium Chloride 10 g/l

Liquid LB mix is 2.5 g per 100 ml solution. Freshly made mix without antibiotics should be autoclaved at 121 C for 20 min or placed in boiling water for 30 min to sterilize.

Pre-mixed powder can be ordered, e.g., (USB/Affymetrix stock #75854).

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