Lysogeny Broth (LB)

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Lysogeny Broth (LB) is made up of:

Casein Peptone 10 g/l
Yeast Extract 5 g/l
Sodium Chloride 10 g/l

Liquid LB mix is 2.5 g per 100 ml solution. Ready made powder can be ordered (USB/Affymetrix stock #75854). Freshly made mix without antibiotics should be autoclaved at 121 C for 20 min or placed in boiling water for 30 min to sterilize.