Difference between revisions of "Pipetting"
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| + | Volumes in the 1 µl – 20 µl range should be transferred with a P20 micropipette. | ||
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| + | Volumes in the 20 µl – 200 µl range should be transferred with a P200 micropipette. | ||
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| + | Volumes in the 200 µl – 1000 µl range should be transferred with a P1000 micropipette. | ||
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| + | In general micropipettes work best in the middle of their range (half of their "number", e.g., 100 µl for a p200). | ||
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| + | Volumes less than 1 µl can be tricky to work with. Use a P10 micropipette instead of a P20 and/or extended pipette tips if you have them. | ||
Revision as of 09:19, 17 July 2018
There are two main but related methods to pipetting, micropipetting for volumes of 1 ml or less and regular pipetting for volumes greater than 1 ml.
regular pipetting
micropipetting
Volumes in the 1 µl – 20 µl range should be transferred with a P20 micropipette.
Volumes in the 20 µl – 200 µl range should be transferred with a P200 micropipette.
Volumes in the 200 µl – 1000 µl range should be transferred with a P1000 micropipette.
In general micropipettes work best in the middle of their range (half of their "number", e.g., 100 µl for a p200).
Volumes less than 1 µl can be tricky to work with. Use a P10 micropipette instead of a P20 and/or extended pipette tips if you have them.
