===Schneider 1972=== Schneider, I. (1972). Cell lines derived from late embryonic stages of //Drosophila melanogaster//. //Development//, 27(2), 353--365. ==Links== * https://dev.biologists.org/content/27/2/353.short * {{private:schneider1972.pdf}} (internal lab link) ==Abstract== The development of three cell lines initiated from the late embryonic stages of //Drosophila melanogaster// is described. The primary cultures consisted of trypsinized fragments from embryos 20–24 h old. The length of time between primary culture and subsequent subculture varied from 8 months for the first line to 3 weeks for the third. All three lines have been maintained //in vitro// for more than a year. The characteristics of each line are given and evidence is presented that at least one line is derived from imaginal disc cells. A few comments on insect tissue culture in general are also made. {{tag>Publication cell_lines Drosophila_melanogaster}}