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+ | This prepares a PCR product from a PCR reaction for Sanger Sequencing. | ||
+ | The ExoSAP reaction " | ||
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+ | * 10 μl PCR product | ||
+ | * 3 μl ExoSAP (which is 1 μl of Exonuclease I and 2 μl of Shrimp Alkaline Phosphatase) | ||
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+ | The mixture is heated to 37 C for 15 min then 80 C for 15 min. This is under the program " | ||
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+ | The result is an " |