View source for LB Agar Plates

LB Agar is made of LB broth with agar added: 
:[[Casein Peptone]] 10 g/l
:[[Yeast Extract]] 5 g/l
:[[Sodium Chloride]] 10 g/l
:[[Agar]] 15 g/l 

The LB/agar is 4 g per 100 ml solution. Freshly made mix without antibiotics should be autoclaved at 121 C for 20 min or placed in boiling water for 30 min to sterilize. Initially the agar will not be dissolved well and will require some stirring to get a clear solution for [[pouring plates]]. Antibiotics (such as [[Ampicillin]]) if used, should not be added until after the media has cooled to less than 60 C, then added, stirred, and poured before the agar sets. 

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