View source for Overlay Plates

Overlay plates can be used to get an even lawn of bacterial growth.  The top LB/agar is made at half concentration, 2 g per 100 ml solution, compared to standard [[LB Agar Plates]].  The bacteria are added only when the mixture has cooled to 45 C.  They must then be immediately stirred (this can be done by [[pipetting]]) and poured on a pre-warmed (37 C) [[LB Agar Plates]].

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