Overlay Plates

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Overlay plates can be used to get an even lawn of bacterial growth. The top LB/agar is made at half concentration, 2 g per 100 ml solution, compared to standard LB Agar Plates. The bacteria are added only when the mixture has cooled to 45 C. They must then be immediately stirred (this can be done by pipetting) and poured on a pre-warmed (37 C) LB Agar Plates.

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