View source for Sanger Sequencing

The sequencing reaction mix for a 10 µl volume is:
:3 µl H<sub>2</sub>O
:3 µl [[ExoSAP Reaction|ExoSAP'd PCR product]]
:4 µl 1 µM [[Sequencing Primer]]

We use the University's sequencing service, which is currently $2.50 per sequence. We put the reaction for each sample in a small eppendorf tube arranged in order on the plate. A form  must be filled out and the plate and form dropped off at Snyder 312.

=How it works=

=Other types of sequencing=

[[Bisulfite sequencing]]

[[Illumina sequencing]]

=What Links Here=
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