Sanger Sequencing

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The sequencing reaction mix for a 10 µl volume is:

3 µl H2O
3 µl ExoSAP'd PCR product
4 µl 1 µM Sequencing Primer

We use the University's sequencing service, which is currently $2.50 per sequence. We put the reaction for each sample in a small eppendorf tube arranged in order on the plate. A form must be filled out and the plate and form dropped off at Snyder 312.

How it works

Other types of sequencing

Bisulfite sequencing

Illumina sequencing

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