Difference between revisions of "Sanger Sequencing"
From Genetics Wiki
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The sequencing reaction mix for a 10 µl volume is: | The sequencing reaction mix for a 10 µl volume is: | ||
:3 µl H<sub>2</sub>O | :3 µl H<sub>2</sub>O | ||
| − | :3 µl ExoSAP'd PCR product | + | :3 µl [[ExoSAP Reaction|ExoSAP'd PCR product]] |
:4 µl 1 µM [[Sequencing Primer]] | :4 µl 1 µM [[Sequencing Primer]] | ||
We use the University's sequencing service, which is currently $2.50 per lane. We put the reaction for each sample in a small eppendorf tube arranged in order on the plate. A form must be filled out and the plate and form dropped off at Snyder 312. | We use the University's sequencing service, which is currently $2.50 per lane. We put the reaction for each sample in a small eppendorf tube arranged in order on the plate. A form must be filled out and the plate and form dropped off at Snyder 312. | ||
Revision as of 09:03, 17 July 2018
The sequencing reaction mix for a 10 µl volume is:
- 3 µl H2O
- 3 µl ExoSAP'd PCR product
- 4 µl 1 µM Sequencing Primer
We use the University's sequencing service, which is currently $2.50 per lane. We put the reaction for each sample in a small eppendorf tube arranged in order on the plate. A form must be filled out and the plate and form dropped off at Snyder 312.
