Revision as of 16:43, 18 July 2018

First Week Ideas

Course Overview

Lab Safety Overview

Bench Number

Name on Label Tape on Lab Coats

Roll Call

Pre-Assessment Questionnaire

Sample Collection Kits

What to Bring Next Week

Pocket Culturing done by instructor to set up motivation for aseptic technique and the role of phage in our environment

Agar Diffusion

Pipetting by Design

Exit Ticket, weigh PbD plates and write amount on board

Second Week Ideas

look at Agar Diffusion plates

look at Pocket Culturing plates

Aseptic Technique

Lab Notebook Guidelines

Phage Isolation from Samples

Reading

Travis, J. (2003). All the world's a phage: Viruses that eat bacteria abound—and surprise. Science News, 164(2), 26-27; http://www.phschool.com/science/science_news/articles/all_worlds_phage.html ; https://scholar.google.com/scholar?cluster=3754690719355237646

Mann, N. H. (2005). The third age of phage. PLoS Biology, 3(5), e182; http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.0030182 ; https://scholar.google.com/scholar?cluster=8983556190157570421

Gilbert, J. A., & Neufeld, J. D. (2014). Life in a world without microbes. PLoS Biology, 12(12), e1002020; http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1002020 ; https://scholar.google.com/scholar?cluster=13377317378749833528

Rohwer, F., Youle, M., Maughan, H., & Hisakawa, N. (2014). Life in our phage world. San Diego: Wholon, 8(8); http://2015phage.org/art.php

Rohwer, F., & Segall, A. M. (2015). In retrospect: a century of phage lessons. Nature, 528(7580), 46; http://hmjaag.de/wp-content/uploads/2015/12/Nature-2015.pdf ; https://scholar.google.com/scholar?cluster=6653877360359958294

Ghorayshi, A. (2015) Mail-Order Viruses Are The New Antibiotics. BuzzFeed, Published Online: https://www.buzzfeed.com/azeenghorayshi/mail-order-viruses-are-the-new-antibiotics

Young, R., & Gill, J. J. (2015). Phage therapy redux—What is to be done?. Science, 350(6265), 1163-1164; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6005185/ ; https://scholar.google.com/scholar?cluster=10822743933444756726

Touchon, M., de Sousa, J. A. M., & Rocha, E. P. (2017). Embracing the enemy: the diversification of microbial gene repertoires by phage-mediated horizontal gene transfer. Current Opinion in Microbiology, 38, 66-73; https://www.researchgate.net/publication/317038382_Embracing_the_enemy_The_diversification_of_microbial_gene_repertoires_by_phage-mediated_horizontal_gene_transfer ; https://scholar.google.com/scholar?cluster=3814489289688138941

Weber, L. (2017). Sewage Saved This Man’s Life. Someday It Could Save Yours. HuffPost, Wellness Section, Published online: https://www.huffingtonpost.com/entry/antibiotic-resistant-superbugs-phage-therapy_us_5913414de4b05e1ca203f7d4

Resources

https://seaphages.org/

https://seaphages.org/forums/

http://phagesdb.org/