SEA-PHAGES
First Week Ideas
- Course Overview
- Lab Safety Overview
- Bench Number
- Name on Label Tape on Lab Coats
- Roll Call
- Pre-Assessment Questionnaire
- Sample Collection Kits
- What to Bring Next Week
- Pocket Culturing
- Agar Diffusion
- Pipetting by Design
- Exit Ticket, weigh PbD plates and write amount on board
Second Week Ideas
- look at Agar Diffusion plates
- look at Pocket Culturing plates
- Aseptic Technique
- Lab Notebook Guidelines
- Phage Isolation from Samples
Reading
Travis, J. (2003). All the world's a phage: Viruses that eat bacteria abound—and surprise. Science News, 164(2), 26-27; http://www.phschool.com/science/science_news/articles/all_worlds_phage.html ; https://scholar.google.com/scholar?cluster=3754690719355237646
Mann, N. H. (2005). The third age of phage. PLoS Biology, 3(5), e182; http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.0030182 ; https://scholar.google.com/scholar?cluster=8983556190157570421
Gilbert, J. A., & Neufeld, J. D. (2014). Life in a world without microbes. PLoS Biology, 12(12), e1002020; http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1002020 ; https://scholar.google.com/scholar?cluster=13377317378749833528
Rohwer, F., Youle, M., Maughan, H., & Hisakawa, N. (2014). Life in our phage world. San Diego: Wholon, 8(8); http://2015phage.org/art.php
Rohwer, F., & Segall, A. M. (2015). In retrospect: a century of phage lessons. Nature, 528(7580), 46; http://hmjaag.de/wp-content/uploads/2015/12/Nature-2015.pdf ; https://scholar.google.com/scholar?cluster=6653877360359958294
Ghorayshi, A. (2015) Mail-Order Viruses Are The New Antibiotics. BuzzFeed, Published Online: https://www.buzzfeed.com/azeenghorayshi/mail-order-viruses-are-the-new-antibiotics
Young, R., & Gill, J. J. (2015). Phage therapy redux—What is to be done?. Science, 350(6265), 1163-1164; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6005185/ ; https://scholar.google.com/scholar?cluster=10822743933444756726
Touchon, M., de Sousa, J. A. M., & Rocha, E. P. (2017). Embracing the enemy: the diversification of microbial gene repertoires by phage-mediated horizontal gene transfer. Current Opinion in Microbiology, 38, 66-73; https://www.researchgate.net/publication/317038382_Embracing_the_enemy_The_diversification_of_microbial_gene_repertoires_by_phage-mediated_horizontal_gene_transfer ; https://scholar.google.com/scholar?cluster=3814489289688138941
Weber, L. (2017). Sewage Saved This Man’s Life. Someday It Could Save Yours. HuffPost, Wellness Section, Published online: https://www.huffingtonpost.com/entry/antibiotic-resistant-superbugs-phage-therapy_us_5913414de4b05e1ca203f7d4
Resources
https://seaphagesphagediscoveryguide.helpdocsonline.com/home
