Difference between revisions of "Sanger Sequencing"
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(Created page with "The sequencing reaction mix for a 10 µl volume is: :3 µl H<sub>2</sub>O :3 µl ExoSAP'd PCR product :4 µl 1 µM sequencing primer We use the University's sequencing servic...") |
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The sequencing reaction mix for a 10 µl volume is: | The sequencing reaction mix for a 10 µl volume is: | ||
:3 µl H<sub>2</sub>O | :3 µl H<sub>2</sub>O | ||
| − | :3 µl ExoSAP'd PCR product | + | :3 µl [[ExoSAP Reaction|ExoSAP'd PCR product]] |
| − | :4 µl 1 µM | + | :4 µl 1 µM [[Sequencing Primer]] |
| − | We use the University's sequencing service | + | We use the University's sequencing service, which is currently $2.50 per sequence. We put the reaction for each sample in a small eppendorf tube arranged in order on the plate. A form must be filled out and the plate and form dropped off at Snyder 312. |
| + | |||
| + | =How it works= | ||
| + | |||
| + | =Other types of sequencing= | ||
| + | |||
| + | [[Bisulfite sequencing]] | ||
| + | |||
| + | [[Illumina sequencing]] | ||
| + | |||
| + | =What Links Here= | ||
| + | {{Special:WhatLinksHere/{{FULLPAGENAME}}}} | ||
Latest revision as of 10:16, 21 July 2018
The sequencing reaction mix for a 10 µl volume is:
- 3 µl H2O
- 3 µl ExoSAP'd PCR product
- 4 µl 1 µM Sequencing Primer
We use the University's sequencing service, which is currently $2.50 per sequence. We put the reaction for each sample in a small eppendorf tube arranged in order on the plate. A form must be filled out and the plate and form dropped off at Snyder 312.
How it works
Other types of sequencing
What Links Here
- Methods (← links)
- ExoSAP Reaction (← links)
