Difference between revisions of "TAE"
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(Created page with "=50X Stock Solution= 242 g TRIS-Base 57.1 ml Acetate 100 ml 0.5 M EDTA Add water up to a 1 L total volume, mix and give it time to dissolve. =1X Working Sol...") |
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| + | Tris-Acetate buffer keeps a solution slightly basic (approximate [[pH]] of 8.3), which keeps the DNA water soluble (the DNA is deprotonated, H<sup>+</sup> is removed). EDTA sequesters (chelates) metal ions which are used (as a co-factor) by many enzymes (particularly divalent [[Magnesium|Mg<sup>2+</sup>]] ions), some of which can degrade the DNA. | ||
| + | |||
=50X Stock Solution= | =50X Stock Solution= | ||
| − | 242 g [[ | + | 242 [[g]] [[Tris]] |
| − | 57.1 ml [[Acetate]] | + | 57.1 [[ml]] [[Acetate]] |
| − | 100 ml 0.5 M [[EDTA]] | + | 100 [[ml]] 0.5 [[M]] [[EDTA]] |
| − | Add water up to a 1 L total volume, mix and give it time to dissolve. | + | Add water up to a 1 [[L]] total volume, mix, and give it time to dissolve. |
=1X Working Solution= | =1X Working Solution= | ||
| − | Add 20 ml of 50X stock to 980 ml of water. | + | Add 20 [[ml]] of [[50X]] stock to 980 [[ml]] of water. |
| + | |||
| + | =Links= | ||
| + | |||
| + | https://www.ncbi.nlm.nih.gov/pubmed/15351274 | ||
| + | |||
| + | https://en.wikipedia.org/wiki/TAE_buffer | ||
| + | |||
| + | =What Links Here= | ||
| + | {{Special:WhatLinksHere/{{FULLPAGENAME}}}} | ||
Latest revision as of 04:37, 28 July 2018
Tris-Acetate buffer keeps a solution slightly basic (approximate pH of 8.3), which keeps the DNA water soluble (the DNA is deprotonated, H+ is removed). EDTA sequesters (chelates) metal ions which are used (as a co-factor) by many enzymes (particularly divalent Mg2+ ions), some of which can degrade the DNA.
50X Stock Solution
Add water up to a 1 L total volume, mix, and give it time to dissolve.
1X Working Solution
Add 20 ml of 50X stock to 980 ml of water.
Links
https://www.ncbi.nlm.nih.gov/pubmed/15351274
https://en.wikipedia.org/wiki/TAE_buffer
What Links Here
- Agarose Gels (← links)
