TAE

Tris-Acetate buffer keeps a solution slightly basic (approximate pH of 8.3), which keeps the DNA water soluble (the DNA is deprotonated, H⁺ is removed). EDTA sequesters (chelates) metal ions which are used (as a co-factor) by many enzymes (particularly divalent Mg²⁺ ions), some of which can degrade the DNA.

50X Stock Solution

242 g Tris

57.1 ml Acetate

100 ml 0.5 M EDTA

Add water up to a 1 L total volume, mix, and give it time to dissolve.

1X Working Solution

Add 20 ml of 50X stock to 980 ml of water.

Links

https://www.ncbi.nlm.nih.gov/pubmed/15351274

https://en.wikipedia.org/wiki/TAE_buffer

What Links Here

• Agarose Gels ‎ (← links)