Difference between revisions of "TAE"

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(Created page with "=50X Stock Solution= 242 g TRIS-Base 57.1 ml Acetate 100 ml 0.5 M EDTA Add water up to a 1 L total volume, mix and give it time to dissolve. =1X Working Sol...")
 
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=50X Stock Solution=
 
=50X Stock Solution=
  
242 g [[TRIS-Base]]
+
242 [[g]] [[TRIS-Base]]
  
57.1 ml [[Acetate]]
+
57.1 [[ml]] [[Acetate]]
  
100 ml 0.5 M [[EDTA]]  
+
100 [[ml]] 0.5 [[M]] [[EDTA]]  
  
Add water up to a 1 L total volume, mix and give it time to dissolve.  
+
Add water up to a 1 [[L]] total volume, mix and give it time to dissolve.  
  
 
=1X Working Solution=
 
=1X Working Solution=
  
Add 20 ml of 50X stock to 980 ml of water.
+
Add 20 [[ml]] of [[50X]] stock to 980 [[ml]] of water.

Revision as of 01:37, 20 July 2018

50X Stock Solution

242 g TRIS-Base

57.1 ml Acetate

100 ml 0.5 M EDTA

Add water up to a 1 L total volume, mix and give it time to dissolve.

1X Working Solution

Add 20 ml of 50X stock to 980 ml of water.