Difference between revisions of "Sanger Sequencing"

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(Created page with "The sequencing reaction mix for a 10 µl volume is: :3 µl H<sub>2</sub>O :3 µl ExoSAP'd PCR product :4 µl 1 µM sequencing primer We use the University's sequencing servic...")
 
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:3 µl H<sub>2</sub>O
 
:3 µl H<sub>2</sub>O
 
:3 µl ExoSAP'd PCR product
 
:3 µl ExoSAP'd PCR product
:4 µl 1 µM sequencing primer
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:4 µl 1 µM [[Sequencing Primer]]
  
We use the University's sequencing service (link), which is currently $2.50 per lane. This form (link) must be filled out and the plate and form dropped off at Snyder 312.  We put the reaction for each sample in a small eppendorf tube arranged in order on a plate.
+
We use the University's sequencing service, which is currently $2.50 per lane. We put the reaction for each sample in a small eppendorf tube arranged in order on the plate. A form  must be filled out and the plate and form dropped off at Snyder 312.

Revision as of 09:02, 17 July 2018

The sequencing reaction mix for a 10 µl volume is:

3 µl H2O
3 µl ExoSAP'd PCR product
4 µl 1 µM Sequencing Primer

We use the University's sequencing service, which is currently $2.50 per lane. We put the reaction for each sample in a small eppendorf tube arranged in order on the plate. A form must be filled out and the plate and form dropped off at Snyder 312.