Difference between revisions of "Sanger Sequencing"
From Genetics Wiki
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:4 µl 1 µM [[Sequencing Primer]] | :4 µl 1 µM [[Sequencing Primer]] | ||
| − | We use the University's sequencing service, which is currently $2.50 per | + | We use the University's sequencing service, which is currently $2.50 per sequence. We put the reaction for each sample in a small eppendorf tube arranged in order on the plate. A form must be filled out and the plate and form dropped off at Snyder 312. |
| + | |||
| + | =What Links Here= | ||
| + | {{Special:WhatLinksHere/{{FULLPAGENAME}}}} | ||
Revision as of 09:06, 17 July 2018
The sequencing reaction mix for a 10 µl volume is:
- 3 µl H2O
- 3 µl ExoSAP'd PCR product
- 4 µl 1 µM Sequencing Primer
We use the University's sequencing service, which is currently $2.50 per sequence. We put the reaction for each sample in a small eppendorf tube arranged in order on the plate. A form must be filled out and the plate and form dropped off at Snyder 312.
What Links Here
- Methods (← links)
- ExoSAP Reaction (← links)
