Sanger Sequencing

The sequencing reaction mix for a 10 µl volume is:

3 µl H₂O

3 µl ExoSAP'd PCR product

4 µl 1 µM Sequencing Primer

We use the University's sequencing service, which is currently $2.50 per lane. We put the reaction for each sample in a small eppendorf tube arranged in order on the plate. A form must be filled out and the plate and form dropped off at Snyder 312.