TAE

Tris-Acetate buffer keeps a solution slightly basic, which keeps DNA water soluble (the DNA is deprotonated, H⁺ is removed). EDTA sequesters metal ions which are used by many enzymes (particularly divalent Mg²⁺ ions).

50X Stock Solution

242 g Tris

57.1 ml Acetate

100 ml 0.5 M EDTA

Add water up to a 1 L total volume, mix and give it time to dissolve.

1X Working Solution

Add 20 ml of 50X stock to 980 ml of water.

Links

https://www.ncbi.nlm.nih.gov/pubmed/15351274

https://en.wikipedia.org/wiki/TAE_buffer

What Links Here

• Agarose Gels ‎ (← links)