Difference between revisions of "DNA Barcoding"
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Latest revision as of 21:17, 3 August 2018
DNA Barcoding can be a useful way to confirm a species identification and is a good teaching tool to introduce students to PCR and working with DNA sequence data.
General Workflow
- Collect sample and record initial data
- Extract DNA
- Optional: Quantify DNA extraction
- Run PCR
- Check PCR product on a gel
- Troubleshoot and optimize PCR conditions if necessary
- ExoSAP the PCR product
- Sanger Sequencing
- Troubleshoot and optimize sequencing if necessary
- Process the sequence trace files into a DNA sequence
- Search online DNA databases for similar sequences
- Submit a record of your data and results to BOLD
