Difference between revisions of "SEA-PHAGES"

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=First Week Ideas=
 
=First Week Ideas=
:[[Lab Safety Overview (SEA-PHAGES)]]
+
:[[Course Overview (SEA-PHAGES)|Course Overview]]
:[[Bench Number]]
+
:[[Lab Safety Overview (SEA-PHAGES)|Lab Safety Overview]]
:[[Name on Label Tape on Lab Coats]]
+
:Bench Number
:[[Pipetting by Design]]
+
:Name on Label Tape on Lab Coats
:[[Agar Diffusion]]
+
:Roll Call
:[[Dollar Culturing]] to set up motivation for aseptic technique and the role of phage in the environment
+
:[[Pre-Assessment Questionnaire]]
 
:[[Sample Collection Kits]]
 
:[[Sample Collection Kits]]
 
:[[What to Bring Next Week]]
 
:[[What to Bring Next Week]]
 +
:[[Pocket Culturing]]
 +
:[[Agar Diffusion]]
 +
:[[Pipetting by Design]]
 +
:Exit Ticket, weigh PbD plates and write amount on board
  
 
=Second Week Ideas=
 
=Second Week Ideas=
 +
:look at Agar Diffusion plates
 +
:look at Pocket Culturing plates
 
:[[Aseptic Technique]]
 
:[[Aseptic Technique]]
 +
:[[Lab Notebook Guidelines]]
 
:[[Phage Isolation from Samples]]
 
:[[Phage Isolation from Samples]]
  
 
=Reading=
 
=Reading=
:Travis, J. (2003). All the world's a phage: Viruses that eat bacteria abound—and surprise. ''Science News'', 164(2), 26-27; http://www.phschool.com/science/science_news/articles/all_worlds_phage.html ; https://scholar.google.com/scholar?cluster=3754690719355237646
+
Travis, J. (2003). All the world's a phage: Viruses that eat bacteria abound—and surprise. ''Science News'', 164(2), 26-27; http://www.phschool.com/science/science_news/articles/all_worlds_phage.html ; https://scholar.google.com/scholar?cluster=3754690719355237646
:Mann, N. H. (2005). The third age of phage. ''PLoS Biology'', 3(5), e182; http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.0030182 ; https://scholar.google.com/scholar?cluster=8983556190157570421
+
 
:Rohwer, F., & Segall, A. M. (2015). In retrospect: a century of phage lessons. ''Nature'', 528(7580), 46; http://hmjaag.de/wp-content/uploads/2015/12/Nature-2015.pdf ; https://scholar.google.com/scholar?cluster=6653877360359958294
+
Mann, N. H. (2005). The third age of phage. ''PLoS Biology'', 3(5), e182; http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.0030182 ; https://scholar.google.com/scholar?cluster=8983556190157570421
:Gilbert, J. A., & Neufeld, J. D. (2014). Life in a world without microbes. ''PLoS Biology'', 12(12), e1002020; http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1002020 ; https://scholar.google.com/scholar?cluster=13377317378749833528
+
 
:Young, R., & Gill, J. J. (2015). Phage therapy redux—What is to be done?. ''Science'', 350(6265), 1163-1164; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6005185/ ; https://scholar.google.com/scholar?cluster=10822743933444756726
+
Gilbert, J. A., & Neufeld, J. D. (2014). Life in a world without microbes. ''PLoS Biology'', 12(12), e1002020; http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1002020 ; https://scholar.google.com/scholar?cluster=13377317378749833528
:Rohwer, F., Youle, M., Maughan, H., & Hisakawa, N. (2014). Life in our phage world. ''San Diego: Wholon'', 8(8); http://2015phage.org/art.php
+
 
:Touchon, M., de Sousa, J. A. M., & Rocha, E. P. (2017). Embracing the enemy: the diversification of microbial gene repertoires by phage-mediated horizontal gene transfer. ''Current Opinion in Microbiology'', 38, 66-73; https://www.researchgate.net/publication/317038382_Embracing_the_enemy_The_diversification_of_microbial_gene_repertoires_by_phage-mediated_horizontal_gene_transfer ; https://scholar.google.com/scholar?cluster=3814489289688138941
+
Rohwer, F., Youle, M., Maughan, H., & Hisakawa, N. (2014). Life in our phage world. ''San Diego: Wholon'', 8(8); http://2015phage.org/art.php
: Weber, L. (2017). Sewage Saved This Man’s Life. Someday It Could Save Yours. ''HuffPost'', Wellness Section, Published online: https://www.huffingtonpost.com/entry/antibiotic-resistant-superbugs-phage-therapy_us_5913414de4b05e1ca203f7d4
+
 
: Ghorayshi, A. (2015) Mail-Order Viruses Are The New Antibiotics. ''BuzzFeed'', Published Online: https://www.buzzfeed.com/azeenghorayshi/mail-order-viruses-are-the-new-antibiotics
+
Rohwer, F., & Segall, A. M. (2015). In retrospect: a century of phage lessons. ''Nature'', 528(7580), 46; http://hmjaag.de/wp-content/uploads/2015/12/Nature-2015.pdf ; https://scholar.google.com/scholar?cluster=6653877360359958294
 +
 
 +
Ghorayshi, A. (2015) Mail-Order Viruses Are The New Antibiotics. ''BuzzFeed'', Published Online: https://www.buzzfeed.com/azeenghorayshi/mail-order-viruses-are-the-new-antibiotics
 +
 
 +
Young, R., & Gill, J. J. (2015). Phage therapy redux—What is to be done?. ''Science'', 350(6265), 1163-1164; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6005185/ ; https://scholar.google.com/scholar?cluster=10822743933444756726
 +
 
 +
Touchon, M., de Sousa, J. A. M., & Rocha, E. P. (2017). Embracing the enemy: the diversification of microbial gene repertoires by phage-mediated horizontal gene transfer. ''Current Opinion in Microbiology'', 38, 66-73; https://www.researchgate.net/publication/317038382_Embracing_the_enemy_The_diversification_of_microbial_gene_repertoires_by_phage-mediated_horizontal_gene_transfer ; https://scholar.google.com/scholar?cluster=3814489289688138941
 +
 
 +
Weber, L. (2017). Sewage Saved This Man’s Life. Someday It Could Save Yours. ''HuffPost'', Wellness Section, Published online: https://www.huffingtonpost.com/entry/antibiotic-resistant-superbugs-phage-therapy_us_5913414de4b05e1ca203f7d4
 +
 
 +
=Resources=
 +
https://seaphages.org/
 +
 
 +
https://seaphages.org/forums/
 +
 
 +
http://phagesdb.org/
 +
 
 +
https://seaphagesphagediscoveryguide.helpdocsonline.com/home
 +
 
 +
=What Links Here=
 +
{{Special:WhatLinksHere/{{FULLPAGENAME}}}}

Latest revision as of 21:16, 4 September 2018

First Week Ideas

Course Overview
Lab Safety Overview
Bench Number
Name on Label Tape on Lab Coats
Roll Call
Pre-Assessment Questionnaire
Sample Collection Kits
What to Bring Next Week
Pocket Culturing
Agar Diffusion
Pipetting by Design
Exit Ticket, weigh PbD plates and write amount on board

Second Week Ideas

look at Agar Diffusion plates
look at Pocket Culturing plates
Aseptic Technique
Lab Notebook Guidelines
Phage Isolation from Samples

Reading

Travis, J. (2003). All the world's a phage: Viruses that eat bacteria abound—and surprise. Science News, 164(2), 26-27; http://www.phschool.com/science/science_news/articles/all_worlds_phage.html ; https://scholar.google.com/scholar?cluster=3754690719355237646

Mann, N. H. (2005). The third age of phage. PLoS Biology, 3(5), e182; http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.0030182 ; https://scholar.google.com/scholar?cluster=8983556190157570421

Gilbert, J. A., & Neufeld, J. D. (2014). Life in a world without microbes. PLoS Biology, 12(12), e1002020; http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1002020 ; https://scholar.google.com/scholar?cluster=13377317378749833528

Rohwer, F., Youle, M., Maughan, H., & Hisakawa, N. (2014). Life in our phage world. San Diego: Wholon, 8(8); http://2015phage.org/art.php

Rohwer, F., & Segall, A. M. (2015). In retrospect: a century of phage lessons. Nature, 528(7580), 46; http://hmjaag.de/wp-content/uploads/2015/12/Nature-2015.pdf ; https://scholar.google.com/scholar?cluster=6653877360359958294

Ghorayshi, A. (2015) Mail-Order Viruses Are The New Antibiotics. BuzzFeed, Published Online: https://www.buzzfeed.com/azeenghorayshi/mail-order-viruses-are-the-new-antibiotics

Young, R., & Gill, J. J. (2015). Phage therapy redux—What is to be done?. Science, 350(6265), 1163-1164; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6005185/ ; https://scholar.google.com/scholar?cluster=10822743933444756726

Touchon, M., de Sousa, J. A. M., & Rocha, E. P. (2017). Embracing the enemy: the diversification of microbial gene repertoires by phage-mediated horizontal gene transfer. Current Opinion in Microbiology, 38, 66-73; https://www.researchgate.net/publication/317038382_Embracing_the_enemy_The_diversification_of_microbial_gene_repertoires_by_phage-mediated_horizontal_gene_transfer ; https://scholar.google.com/scholar?cluster=3814489289688138941

Weber, L. (2017). Sewage Saved This Man’s Life. Someday It Could Save Yours. HuffPost, Wellness Section, Published online: https://www.huffingtonpost.com/entry/antibiotic-resistant-superbugs-phage-therapy_us_5913414de4b05e1ca203f7d4

Resources

https://seaphages.org/

https://seaphages.org/forums/

http://phagesdb.org/

https://seaphagesphagediscoveryguide.helpdocsonline.com/home

What Links Here