SEA-PHAGES

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First Week Ideas

Lab Safety Overview
Bench Number
Name on Label Tape on Lab Coats
Roll Call
Pre-Assessment Questionnaire
Sample Collection Kits
What to Bring Next Week
Agar Diffusion
Pipetting by Design
Exit Ticket, weigh PbD plates and write amount on board

Second Week Ideas

Aseptic Technique
Pocket Culturing to set up motivation for aseptic technique and the role of phage in our environment
Phage Isolation from Samples

Reading

Travis, J. (2003). All the world's a phage: Viruses that eat bacteria abound—and surprise. Science News, 164(2), 26-27; http://www.phschool.com/science/science_news/articles/all_worlds_phage.html ; https://scholar.google.com/scholar?cluster=3754690719355237646
Mann, N. H. (2005). The third age of phage. PLoS Biology, 3(5), e182; http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.0030182 ; https://scholar.google.com/scholar?cluster=8983556190157570421
Gilbert, J. A., & Neufeld, J. D. (2014). Life in a world without microbes. PLoS Biology, 12(12), e1002020; http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1002020 ; https://scholar.google.com/scholar?cluster=13377317378749833528
Rohwer, F., Youle, M., Maughan, H., & Hisakawa, N. (2014). Life in our phage world. San Diego: Wholon, 8(8); http://2015phage.org/art.php
Rohwer, F., & Segall, A. M. (2015). In retrospect: a century of phage lessons. Nature, 528(7580), 46; http://hmjaag.de/wp-content/uploads/2015/12/Nature-2015.pdf ; https://scholar.google.com/scholar?cluster=6653877360359958294
Ghorayshi, A. (2015) Mail-Order Viruses Are The New Antibiotics. BuzzFeed, Published Online: https://www.buzzfeed.com/azeenghorayshi/mail-order-viruses-are-the-new-antibiotics
Young, R., & Gill, J. J. (2015). Phage therapy redux—What is to be done?. Science, 350(6265), 1163-1164; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6005185/ ; https://scholar.google.com/scholar?cluster=10822743933444756726
Touchon, M., de Sousa, J. A. M., & Rocha, E. P. (2017). Embracing the enemy: the diversification of microbial gene repertoires by phage-mediated horizontal gene transfer. Current Opinion in Microbiology, 38, 66-73; https://www.researchgate.net/publication/317038382_Embracing_the_enemy_The_diversification_of_microbial_gene_repertoires_by_phage-mediated_horizontal_gene_transfer ; https://scholar.google.com/scholar?cluster=3814489289688138941
Weber, L. (2017). Sewage Saved This Man’s Life. Someday It Could Save Yours. HuffPost, Wellness Section, Published online: https://www.huffingtonpost.com/entry/antibiotic-resistant-superbugs-phage-therapy_us_5913414de4b05e1ca203f7d4

Resources

https://seaphages.org/
https://seaphages.org/forums/
http://phagesdb.org/