Difference between revisions of "Sanger Sequencing"
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Revision as of 08:59, 17 July 2018
The sequencing reaction mix for a 10 µl volume is:
- 3 µl H2O
- 3 µl ExoSAP'd PCR product
- 4 µl 1 µM sequencing primer
We use the University's sequencing service (link), which is currently $2.50 per lane. This form (link) must be filled out and the plate and form dropped off at Snyder 312. We put the reaction for each sample in a small eppendorf tube arranged in order on a plate.
