Difference between revisions of "TAE"

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(50X Stock Solution)
Line 1: Line 1:
 
=50X Stock Solution=
 
=50X Stock Solution=
  
242 [[g]] [[TRIS-Base]]
+
242 [[g]] [[TRIS]]
  
 
57.1 [[ml]] [[Acetate]]
 
57.1 [[ml]] [[Acetate]]
Line 7: Line 7:
 
100 [[ml]] 0.5 [[M]] [[EDTA]]  
 
100 [[ml]] 0.5 [[M]] [[EDTA]]  
  
Add water up to a 1 [[L]] total volume, mix and give it time to dissolve.  
+
Add water up to a 1 [[L]] total volume, mix and give it time to dissolve.
  
 
=1X Working Solution=
 
=1X Working Solution=
  
 
Add 20 [[ml]] of [[50X]] stock to 980 [[ml]] of water.
 
Add 20 [[ml]] of [[50X]] stock to 980 [[ml]] of water.

Revision as of 05:23, 20 July 2018

50X Stock Solution

242 g TRIS

57.1 ml Acetate

100 ml 0.5 M EDTA

Add water up to a 1 L total volume, mix and give it time to dissolve.

1X Working Solution

Add 20 ml of 50X stock to 980 ml of water.