Difference between revisions of "Overlay Plates"
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(Created page with "Overlay plates can be used to get an even lawn of bacterial growth. The top LB/agar is made at half concentration, 2 g per 100 ml solution, compared to standard LB Agar Pla...") |
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Overlay plates can be used to get an even lawn of bacterial growth. The top LB/agar is made at half concentration, 2 g per 100 ml solution, compared to standard [[LB Agar Plates]]. The bacteria are added only when the mixture has cooled to 45 C. They must then be immediately stirred (this can be done by [[pipetting]]) and poured on a pre-warmed (37 C) [[LB Agar Plates]]. | Overlay plates can be used to get an even lawn of bacterial growth. The top LB/agar is made at half concentration, 2 g per 100 ml solution, compared to standard [[LB Agar Plates]]. The bacteria are added only when the mixture has cooled to 45 C. They must then be immediately stirred (this can be done by [[pipetting]]) and poured on a pre-warmed (37 C) [[LB Agar Plates]]. | ||
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Latest revision as of 08:41, 17 July 2018
Overlay plates can be used to get an even lawn of bacterial growth. The top LB/agar is made at half concentration, 2 g per 100 ml solution, compared to standard LB Agar Plates. The bacteria are added only when the mixture has cooled to 45 C. They must then be immediately stirred (this can be done by pipetting) and poured on a pre-warmed (37 C) LB Agar Plates.
