Difference between revisions of "Agarose Gels"
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(→Simple 1% Ethidium Bromide (EtBr) Gel) |
(→Simple 1% Ethidium Bromide (EtBr) Gel) |
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100 [[mL]] [[1X]] [[TAE]] | 100 [[mL]] [[1X]] [[TAE]] | ||
| − | Mix agarose into the TAE | + | Mix agarose into the TAE buffer in a microwaveable clear container. Microwave in 30 s to 1 min intervals, handle the container with a silicone glove, swirling between each step to mix the agarose to help it dissolve. Be careful of flash boiling and the hot liquid suddenly splattering or spilling over. |
Once the agarose is fully dissolved and clear let it cool a few minutes and then add EtBr and swirl to mix. | Once the agarose is fully dissolved and clear let it cool a few minutes and then add EtBr and swirl to mix. | ||
| Line 11: | Line 11: | ||
2.5 [[μl]] [[EtBr]] | 2.5 [[μl]] [[EtBr]] | ||
| − | = | + | ...pouring the gel into a mold ... |
| + | |||
| + | =Notes= | ||
Different gel concentrations ... | Different gel concentrations ... | ||
[[Gel Stains]] (potentially optional) ... | [[Gel Stains]] (potentially optional) ... | ||
| + | |||
| + | =Used For= | ||
| + | |||
| + | [[Electrophoresis]] | ||
| + | |||
| + | =What Links Here= | ||
| + | {{Special:WhatLinksHere/{{FULLPAGENAME}}}} | ||
Latest revision as of 04:32, 28 July 2018
Simple 1% Ethidium Bromide (EtBr) Gel
Mix agarose into the TAE buffer in a microwaveable clear container. Microwave in 30 s to 1 min intervals, handle the container with a silicone glove, swirling between each step to mix the agarose to help it dissolve. Be careful of flash boiling and the hot liquid suddenly splattering or spilling over.
Once the agarose is fully dissolved and clear let it cool a few minutes and then add EtBr and swirl to mix.
...pouring the gel into a mold ...
Notes
Different gel concentrations ...
Gel Stains (potentially optional) ...
