Difference between revisions of "LB Agar Plates"
From Genetics Wiki
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:[[Agar]] 15 g/l | :[[Agar]] 15 g/l | ||
| − | The LB/agar is 4 g per 100 ml solution. | + | The LB/agar is 4 g per 100 ml solution. Freshly made mix without antibiotics should be autoclaved at 121 C for 20 min or placed in boiling water for 30 min to sterilize. Initially the agar will not be dissolved well and will require some stirring to get a clear solution for [[pouring plates]]. |
=What Links Here= | =What Links Here= | ||
{{Special:WhatLinksHere/{{FULLPAGENAME}}}} | {{Special:WhatLinksHere/{{FULLPAGENAME}}}} | ||
Revision as of 08:34, 17 July 2018
LB Agar is made of LB broth with agar added:
- Casein Peptone 10 g/l
- Yeast Extract 5 g/l
- Sodium Chloride 10 g/l
- Agar 15 g/l
The LB/agar is 4 g per 100 ml solution. Freshly made mix without antibiotics should be autoclaved at 121 C for 20 min or placed in boiling water for 30 min to sterilize. Initially the agar will not be dissolved well and will require some stirring to get a clear solution for pouring plates.
What Links Here
- Methods (← links)
- Overlay Plates (← links)
- Agar Diffusion (← links)
