Difference between revisions of "Agar Diffusion"
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=Red Food Coloring= | =Red Food Coloring= | ||
| − | This was done with a single drop of food coloring containing FD&C Red 3 and Red 40. Be careful not to disturb the plate and cause the drop to run until it is | + | This was done with a single drop of food coloring containing FD&C Red 3 and Red 40. Be careful not to disturb the plate and cause the drop to run until it is completely absorbed into the media. |
[[File:Reddropagarose.jpg|200px|thumb|left|Starting Drop]] | [[File:Reddropagarose.jpg|200px|thumb|left|Starting Drop]] | ||
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[[File:reddropdiffusion5.jpg|200px|thumb|left|Two days later, inverted]] | [[File:reddropdiffusion5.jpg|200px|thumb|left|Two days later, inverted]] | ||
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| + | =Loading Dye= | ||
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| + | This was done with 5 ul of gel loading dye (normally used for electrophoresis). Be careful not to disturb the plate and cause the drop to run until it is completely absorbed into the media. The loading dye quickly became less clear than the food coloring. | ||
=Notes= | =Notes= | ||
Revision as of 07:46, 21 July 2018
A simple demonstration of diffusion through agar media to visually illustrate that viruses diffuse through plate media.
Red Food Coloring
This was done with a single drop of food coloring containing FD&C Red 3 and Red 40. Be careful not to disturb the plate and cause the drop to run until it is completely absorbed into the media.
After sitting overnight the liquid was completely absorbed into the media and the plate can be inverted.
Loading Dye
This was done with 5 ul of gel loading dye (normally used for electrophoresis). Be careful not to disturb the plate and cause the drop to run until it is completely absorbed into the media. The loading dye quickly became less clear than the food coloring.
Notes
Carbon black has a particle size closer to that of a virus and might be more appropriate to model viral particle diffusion.
What Links Here
- SEA-PHAGES (← links)
