LB Agar Plates

LB Agar is made of LB broth with agar added:

Casein Peptone 10 g/l

Yeast Extract 5 g/l

Sodium Chloride 10 g/l

Agar 15 g/l

The LB/agar is 4 g per 100 ml solution. Freshly made mix without antibiotics should be autoclaved at 121 C for 20 min or placed in boiling water for 30 min to sterilize. Initially the agar will not be dissolved well and will require some stirring to get a clear solution for pouring plates.

What Links Here

• Methods ‎ (← links)
• Overlay Plates ‎ (← links)
• Agar Diffusion ‎ (← links)